Ushbu to'plam nuklein kislotani in vitroda sifatli aniqlash uchun ishlatiladiInson zardobida maymunpox virusi, lezyon ekssudati namunalari vaqoraqo'tir namunalari.Primer to'plamlari va FAM etiketli problar ishlab chiqilganMonkeypox virusi, inson RNase P genini aniq aniqlash uchunsinov namunasi bilan bir vaqtda olinadigan ichki qismni ta'minlaydinuklein ekstraktsiya jarayoni va reagentni tasdiqlash uchun nazorat qilishyaxlitlik.Insonning RNase P geniga qaratilgan prob VIC bilan belgilangan.
| Komponentlar | 48 Testlar/to'plam | 96 testlar/to'plam |
| PCR reaksiya buferi | 672 mL × 1 naycha | 672 mL × 2 naycha |
| PCR ferment aralashmasi | 50 mkL × 1 naycha | 100 mkL × 1 naycha |
| Ijobiy nazorat | 100 mkL × 1 naycha | 200 mkl × 1 naycha |
| Salbiy nazorat | 100 mkL × 1 naycha | 200 mkl × 1 naycha |
1. Sezuvchanlik: 200 nusxa/ml.
2. O'ziga xoslik: Enterovirus (EV), Qizamiq bilan o'zaro reaktsiya yo'qvirus (MV), qizilcha virusi (RV), varikella-zoster virusi (VZV),Dang virusi (DenV), insonParvovirus B19 (HPVB19),Epstein-barr virusi (EBv), inson gerpes virusi 6 (HHV-6)
3. Aniqlik: CV ≤ 5%.
Haqiqiy vaqtda PCR tizimi: Diagenex AL, ABI 7500, ViiATM 7, QuantStudio 5, QuantStudio 6/7 pro, QuantStudio 6/7 flex, Agilent Mx3000P/3005P, Rotor-GeneTM 6000/CFRad Touch, Bio ，Hongshi SLAN-96S/96P，AGS8830, AGS4800